Fig. 2 | Laboratory Investigation

Fig. 2

From: Mutation screening using formalin-fixed paraffin-embedded tissues: a stratified approach according to DNA quality

Fig. 2

Alternative allele depth (AAD) as an effective parameter in dichotomy between reproducible and non-reproducible variants. In general, the AAD value for non-reproducible variants is low, particularly in DNA samples that support PCR of 300 bp or more genomic fragments, with mean plus 2 SD being less than 15 AAD. To ensure highly specific mutation detection, a cut-off value of 20 AAD is used for dichotomy between reproducible and non-reproducible variants.

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