Fig. 6 | Laboratory Investigation

Fig. 6

From: S100A4 protects mice from high-fat diet-induced obesity and inflammation

Fig. 6

Effects of overexpressed S100A4 on the expression of Akt in 3T3-L1 adipocytes. a 3T3-L1 cells were transfected with either a plasmid overexpressing S100A4 (pEGFP-S100A4) or a control plasmid (pEGFP). Insulin signaling was quantified by phosphorylation of the downstream effector Akt. b Quantification of relative phospho-Akt by Image J. c qPCR analysis of lipogenic gene expression. d Relative mRNA expression of inflammatory genes. *P < 0.05, **P < 0.01

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