Fig. 3
From: An in vivo model for thyroid regeneration and folliculogenesis
Signaling in NKX2-1-overexpressing cells. a GFP immunofluorescence to determine the efficiency of NKX2-1 overexpression in the presence or absence of doxycycline (DOX, 1 µg/ml) in SPTL cells. b Western blotting for thyroid and epithelial differentiation markers in SPTL cells cultured for 2 days under 0.1% FBS without (−) and with (+) DOX (1 µg/ml). Thy: mouse thyroid follicular cells. β-actin was used as a loading control. c Cell morphology of SPTL cells cultured under DOX. Images were taken after 3 days culture under 2% FBS in the absence or presence of DOX (3 µg/ml). d Western blotting for DOX dose-dependent expression of NKX2-1, ERK, pERK, AKT, and pAKT in SPTL cells. Cells were cultured under 0.1% FBS and treated with DOX for 2 days. 10% FBS for 1.3 hours was used as positive control for pAKT. α-tubulin was used as a loading control. e Immunofluorescence for NKX2-1 and pAKT in a zone of cells located near the tracheal cartilage and muscle in Nkx2-1(fl/fl);TPO-cre mouse. Arrows: cells expressing both pAKT and NKX2-1
