Fig. 4

Inhibition of Nlrp3-related inflammation by glyburide attenuates the OGD-induced and CPB-induced pyroptosis in AM. NR8383 AM were pre-treated with, or without, glyburide for 30 min and exposed to OGD. In addition, rats were randomized and treated intraperitoneally with a single dose of 50 mg/kg glyburide 1 h before the CPB or sham surgery. After 6 h, the levels of Nlrp3 expression and AM pyroptosis were determined. Data are representative images or expressed as the mean ± SEM of each group (n = 3 per group for cell assays; n = 6 per group of rats) from three separate experiments. a, b Quantitative RT-PCR and Western blot analysis of the levels of Nlrp3 expression in AM and in the lungs. c, h Western blot analysis of the relative levels of pyroptosis-related protein expression in AM and in the lungs, respectively. d, i The levels of caspase-1 activity in AM and the lungs, respectively. e The levels of IL-1β in the cell supernatants. f, j The frequency of pyroptotic AM and BALF AM. g The ASC foci formation in AM was analyzed by immunofluorescence (magnification ×760). **p < 0.01 or *p < 0.05 vs. the control group; ^##p < 0.01 vs. the OGD+ glyburide (gly) group or the CPB+ glyburide (gly) group