Fig. 5 | Laboratory Investigation

Fig. 5

From: Characterization of a murine nonalcoholic steatohepatitis model induced by high fat high calorie diet plus fructose and glucose in drinking water

Fig. 5The alternative text for this image may have been generated using AI.

Insulin resistance status in different groups. A, B Intraperitoneal glucose tolerance test (IGTT) at 8 (A) and 16 (B) weeks. C, D Serum insulin levels and index of homeostasis model assessment of insulin resistance (HOMA-IR) at 8 (C) and 16 (D) weeks. E, F qRT-PCR analysis of INSR, IRS-1, and IRS-2 at 8 (E) and 16 (F) weeks. A–F n = 3 for 8 weeks and n = 5 for 16 weeks. G Relative pIRS-1 and pAkt protein levels were determined by western blot analysis at 8 and 16 weeks (n = 3). *p < 0.05 and **p < 0.01 compared to the controls. ap < 0.05 and aap < 0.01 compared to HF/G. bp < 0.05 and bbp < 0.01 compared to HFCD. INSR insulin receptor, IRS-1 insulin receptor substrate-1, IRS-2 insulin receptor substrate-2, Akt PKB (protein kinase B). pIRS1 phosphorylation of IRS-1 at serine 307, pAkt phosphorylation of Akt-1 at serine 473

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