Fig. 5 | Laboratory Investigation

Fig. 5

From: Characterization of a murine nonalcoholic steatohepatitis model induced by high fat high calorie diet plus fructose and glucose in drinking water

Fig. 5

Insulin resistance status in different groups. A, B Intraperitoneal glucose tolerance test (IGTT) at 8 (A) and 16 (B) weeks. C, D Serum insulin levels and index of homeostasis model assessment of insulin resistance (HOMA-IR) at 8 (C) and 16 (D) weeks. E, F qRT-PCR analysis of INSR, IRS-1, and IRS-2 at 8 (E) and 16 (F) weeks. A–F n = 3 for 8 weeks and n = 5 for 16 weeks. G Relative pIRS-1 and pAkt protein levels were determined by western blot analysis at 8 and 16 weeks (n = 3). *p < 0.05 and **p < 0.01 compared to the controls. ap < 0.05 and aap < 0.01 compared to HF/G. bp < 0.05 and bbp < 0.01 compared to HFCD. INSR insulin receptor, IRS-1 insulin receptor substrate-1, IRS-2 insulin receptor substrate-2, Akt PKB (protein kinase B). pIRS1 phosphorylation of IRS-1 at serine 307, pAkt phosphorylation of Akt-1 at serine 473

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