Fig. 7 | Laboratory Investigation

Fig. 7

From: Characterization of a murine nonalcoholic steatohepatitis model induced by high fat high calorie diet plus fructose and glucose in drinking water

Fig. 7

Expression levels of critical enzymes and transcription factors involved in fatty acid synthesis and β-oxidation. A, B Total protein (A) and nuclear protein (B) levels of SREBP-1c, ChREBP, FXR, LXR-α, PPAR-α, PPAR-γ, and Fasn (total protein) at 8 and 16 weeks were analyzed by western blot analysis (n = 3). C, D mRNA levels of Fasn, ACC-β, SCD-1, CPT-1, and ACOX-1 at 8 (C) and 16 (D) weeks were determined by quantitative RT-PCR analysis (n = 3). *p < 0.05 and **p < 0.01 compared to the controls. SREBP-1c sterol regulatory element binding protein-1c, ChREBP carbohydrate response element binding protein, FXR farnesoid X receptor, LXR-α liver X receptor-α, PPAR-α peroxisome proliferator-activated receptor-α, PPAR-γ peroxisome proliferators-activated receptor-γ, Fasn fatty acid synthase, TBP TATA-binding protein as a nuclear protein, ACC-β acetyl-CoA carboxylase-β, SCD-1 stearoyl-CoA desaturase-1, CPT-1 carnitine palmitoyltransferase-1, ACOX-1 acyl-CoA oxidase-1

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