Fig. 8 | Laboratory Investigation

Fig. 8

From: Characterization of a murine nonalcoholic steatohepatitis model induced by high fat high calorie diet plus fructose and glucose in drinking water

Fig. 8The alternative text for this image may have been generated using AI.

Inflammasome activation in mice under different diet feeding. A, B Representative confocal micrographs of ALB, α-SMA, and CD163 with NLRP-1 (A) and NLRP-3 co-immunofluorescent staining (B) at 16 weeks. For a–d, ALB was stained in red and NLRP1/3 was stained in green; for e–h, α-SMA was stained in green and NLRP1/3 was stained in red; for i–l, CD163 was stained in green and NLRP1/3 was in red. Images were taken at original magnification (×400). Scale bars = 50 μm. C Western blot analysis of NLRP-1, NLRP-3, cleaved IL-1β, and cleaved caspase-1 protein levels at 8 and 16 weeks (n = 3). NLRP1 NOD-like receptor-1, NLRP3 NOD-like receptor-3, ALB albumin, α-SMA smooth muscle α-actin, CD163 cluster of differentiation 163

Back to article page