Fig. 2
From: Mast cells contribute to Enterovirus 71 infection-induced pulmonary edema in neonatal mice
EV71 infection caused pulmonary edema and release of inflammatory cytokines. Three-day-old mice were inoculated with ZZ1350 strain or same volume of RD cell culture supernatants and lung tissues were harvested at 7 dpi. a Representative image of mock and EV71-infected lungs, and ratio of wet lung weight/body weight from mice with EV71 infection (n = 6) and controls (n = 5). Lm, EG-VEGF and NA are known as the indicators of pulmonary edema. Lm (b) of mice lungs (n = 5 for mock and n = 6 for EV71) was calculated based on 10 randomly selected fields in each section at ×10 magnification with two crossed test lines. Levels of EG-VEGF (c) and NA (d) in mice lungs (n = 5 for mock and n = 6 for EV71) were detected using ELISA kits. e H&E, Masson’s Trichrome and PAS stained lungs from mock (n = 5) and EV71 infection (n = 5), which was evaluated by histology score. f Inflammatory cytokines in lung lysates from mock (n = 4) and EV71-infected mice (n = 4) were measured by a Quantibody® Mouse Inflammation Array I kit. g Intensity of inflammatory cytokines expression in lung lysates from mock and EV71-infected mice. g The name and location of inflammatory cytokines list. h Relative quantitative expression levels of inflammatory cytokines in lung lysates from mock- and EV71-infected mice. The data are expressed as means ± SEM. *P < 0.05, vs. Mock; **P < 0.01, vs. Mock; ***P < 0.001, vs. Mock
