Fig. 3

Effect of APOE deficiency on bone formation and resorption by dynamic histomorphometry. Reduced bone formation and increased osteoclast resorption in the lumbar vertebrae of APOE deficient mice that were fed WTD mice relatively to C57BL/6 WTD. a, b Calcein labeled surfaces (green lines) and calcein double-labeling (yellow arrows) are more intense in the vertebrae of the C57BL/6 WTD compared to the APOE knockout mice (original magnification 20×). c, d TRAP stain showed increased TRAP activity in the APOE deficient compared to C57BL/6 WTD mice (the TRAP positive cells/areas are colored red) (original magnification 10×)