Fig. 5 | Laboratory Investigation

Fig. 5

From: Western-type diet differentially modulates osteoblast, osteoclast, and lipoblast differentiation and activation in a background of APOE deficiency

Fig. 5

Western blots of key bone and lipoblast regulatory proteins in APOE deficient and wild type mice. a Western blot results from RUNX2 and PPARγ analyses, normalized to actin expression. The protein levels of both the osteoblastic (RUNX2) and the lipoblastic regulator (PPARγ) were significantly reduced to the APOE deficient mice challenged with WTD, in comparison to the control mice. b Determination of intracellular RUNX2 and PPARγ protein levels in WΒΜCs by flow cytometry presented as median fluorescent intensities (MFI). Histograms of count and intensity are shown on the left; summaries of quantitative data on mean PPARγ and RUNX2 antibody labeling are depicted on the right

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