Fig. 1 | Laboratory Investigation

Fig. 1

From: The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo

Fig. 1

Confirmation of SSH1 knockout by RT-qPCR and western blot. a The aortas of Ssh1+/+ and Ssh1−/− mice were harvested for mRNA. SSH1 and SSH2 mRNAs were measured by RT-qPCR and corrected by housekeeping gene PGK. Values are represented as fold change vs. Ssh1+/+ (*p < 0.0001, n = 4 mice per group). Protein was harvested from the aortas of Ssh1+/+ and Ssh1−/− mice, analyzed by western blot and probed with antibodies to b SSH1 and actin (representative blot, n = 2) or c p-Cofilin, and Cofilin. Measurable changes in protein expression were quantified using densiometric analysis (*p < 0.006, n = 5–8 mice per group)

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