Fig. 5
Loss of SSH1 exacerbates angiotensin II-induced increases in aortic wall thickness and fibrotic matrix deposition. Mice were treated with Ang II or saline for 14 days. Aortas were harvested, embedded in paraffin, cut into 5 μm sections, and stained with Masson’s Trichrome. a Images were scanned using a ×40 objective (scale bar = 80 μm) as well as a ×10 objective (scale bar = 295 μm). Ang II media changes in aortic structure were evaluated by measuring changes in the b media-to-lumen ratio of Ssh1+/+ and Ssh1−/− mice using ImageJ software. (*p = 0.04, n = 3–5; **p < 0.001, n = 3–6 mice per group; ***p < 0.001, n = 3–6 mice per group). c Medial fibrotic matrix deposition (*p < 0.001, n = 3–4; **p < 0.001, n = 3–5 mouse per group; ***p < 0.001, n = 3–5 mice per group) was quantified by ImageJ
