Fig. 1
From: An immunohistochemical approach to detect oncogenic CTNNB1 mutations in primary neoplastic tissues
Development of an immunohistochemical assay for the detection of mutant β-catenin using the HCT 116 cell line. A hematoxylin and eosin (H&E)-stained section is shown (a). The serine 45 CTNNB1 mutation harbored by the HCT116 cell line is confirmed via Sanger sequencing (b). Strong cytoplasmic/nuclear accumulation of β-catenin with the antibody against total β-catenin is seen along with the lack of staining with the anti-ABC (active β-catenin) antibody via immunohistochemistry (c, d). Scale bars, 100 μm
