Fig. 2 | Laboratory Investigation

Fig. 2

From: MEF2B is a member of the BCL6 gene transcriptional complex and induces its expression in diffuse large B-cell lymphoma of the germinal center B-cell-like type

Fig. 2

a Cell cycle analysis demonstrates G1, S, and G2 phases in MEF2B-deficient (SU-DHL-2) and MEF2B-expressing (SU-DHL-4) cell lines. Cell populations represent each phase of cell cycle for SU-DHL-2 and SU-DHL-4 cell lines. Data represent the mean±standard deviation (SD) (n = 3). b Flow cytometry analysis of apoptosis demonstrates the portion of annexin V-stained cells in SU-DHL-2 and SU-DHL-4 cell lines. Apoptotic cells are represented in the lower right corner. Data are representative of three independent experiments performed separately. c Western blot analysis demonstrates that the knockdown of MEF2B by its specific siRNA in SU-DHL-4, SU-DHL-5, and SU-DHL-6 cell lines is associated with the inhibition of the expression of BCL6 and CD10 as well as the phosphorylation of ERK proteins. Actin was used as an internal control for loading and transfer. Data are representative of three independent experiments. d MTT assay demonstrates in a time-course experiment the inhibition of the growth rate of SU-DHL-4, SU-DHL-5, and SU-DHL-6 cell lines by the knockdown of MEF2B by its specific siRNA. The values are expressed as the means ± SD of three independent experiments performed in duplicate

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