Fig. 5

Immunofluorescent staining of a HFN4a, a hepatocyte specific transcription factor, in red, phophohistone H3 (PHH3), a marker of mitotic cells, in green and nucleus in blue (DAPI) and b Lyve-1, a endothelial cell marker, in red, PHH3, in green and nucleus in blue (DAPI) in PVLT liver 3 days after first procedure confirming that hepatocytes (DAPI-HNF4α + cells) but not LSEC (Lyve-1 + cells) undergo cell proliferation. Bar size 10 µm