Table 4 Effects of IL-10 on gene expression in the mouse kidney

From: Protective effect and mechanism of IL-10 on renal ischemia–reperfusion injury

 

5 h

24 h

48 h

120 h

 

WT

IL-10 KO

WT

IL-10 KO

WT

IL-10 KO

WT

IL-10 KO

IL-6

1229.0 ± 265.2

1621.0 ± 310.2

72.3 ± 33.0

1037.0 ± 613.5*

28.7 ± 3.8

38.1 ± 11.1

36.8 ± 8.0

21.2 ± 6.3

IL-18

1.0 ± 0.08

0.8 ± 0.08

1.7 ± 0.2

2.4 ± 0.1**

0.7 ± 0.07

0.6 ± 0.05

0.8 ± 0.03

1.3 ± 0.1*

TNF-α

2.4 ± 0.1

2.1 ± 0.2

2.2 ± 0.2

3.6 ± 0.4*

2.4 ± 0.1

2.0 ± 0.2

2.2 ± 0.5

3.2 ± 0.4

IL-1β

1.8 ± 0.4

2.2 ± 0.3

0.9 ± 0.1

2.3 ± 0.5*

1.0 ± 0.07

1.1 ± 0.1

0.7 ± 0.1

1.6 ± 0.3

RANTES

0.9 ± 0.06**

0.5 ± 0.05

1.6 ± 0.1

3.6 ± 0.9*

1.9 ± 0.3

1.0 ± 0.4

1.5 ± 0.3

4.5 ± 0.7*

  1. Cytokine and chemokine gene expression was measured at 5 h (n = 6), 24 h (n = 6), 48 h (n = 6), and 120 h (n = 5) after renal IRI in WT and IL-10 KO mice by real-time RT-PCR. In each experiment, the expression levels were normalized to the expression of 18S rRNA and are expressed relative to control mice. The data are mean fold-increase ± SEM
  2. *p < 0.05, **p < 0.01, WT vs. IL-10 KO mice at 5, 24, and 120 h
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