Table 1 The primer sequences of targeted RNA
Gene primer | Species | Sequence (5′–3′) | |
|---|---|---|---|
Icam-1 | Homo | Forward | GGCAAGAACCTTACCCTACG |
Reverse | AGTGCGGCACGAGAAATT | ||
Vcam-1 | Homo | Forward | GACTATTTTTCTCCTGAGCTTCTCG |
Reverse | GACTATTTTTCTCCTGAGCTTCTCG | ||
Occludin | Homo | Forward | GGAACACATTTATGATGAGCAG |
Reverse | GGAACACATTTATGATGAGCAG | ||
Zo-1 | Homo | Forward | AGCCATTCCCGAAGGAGTTGAG |
Reverse | ATCACAGTGTGGTAAGCGCAGC | ||
β-actin | Homo | Forward | CTACCTCATGAAGATCCTCACCGA |
Reverse | TTCTCCTTAATGTCACGCACGATT | ||
Il-6 | Mus | Forward | TCACAGAAGGAGTGGCTAAGGACC |
Reverse | ACGCACTAGGTTTGCCGAGTAGAT | ||
Cxcl1 | Mus | Forward | TAGTAGAAGGGTGTTGTGCGAAA |
Reverse | AATGTCCAAGGGAAGCGTCA | ||
Cxcl2 | Mus | Forward | CACTCTCAAGGGCGGTCAAA |
Reverse | GCTCCTCCTTTCCAGGTCAGTTA | ||
Glp-1r | Mus | Forward | GGTGGATGTAGTTCCTGGTGC |
Reverse | CTTTCTTTCTCCGCCTTGGTC | ||
Tlr-4 | Mus | Forward | GGAACAAACAGCCTGAGACAC |
Reverse | CAAGGGATAAGAACGCTGAGAA | ||
Mif | Mus | Forward | CCCACGTTGGCAGCGTTCATG |
Reverse | CCGGCAAGCCCGCACAGTACA | ||
β-actin | Mus | Forward | CTCTCCCTCACGCCATC |
Reverse | ACGCACGATTTCCCTCTC |
