Fig. 1 | Laboratory Investigation

Fig. 1

From: Osteoblast lineage-specific cell-surface antigen (A7) regulates osteoclast recruitment and calcification during bone remodeling

Fig. 1

Expression of A7 antigen on cell surface of osteoblastic cell line ROS17/2.8. CL#7 cells were cultured in 35 mm glass-bottom dishes (1.8 x 104 cells/ml, 3.6 x 104 cells/dish) for 4 days. Unfixed cells were stained with A7 MAb (a, b) or isotype IgG (c) as the the primary antibody as described in Materials and Methods. After rinsing, fixing and blocking, cells were treated with anti-mouse IgG conjugated with Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). a A7 antigen expression in areas of lower cell density detected by A7 MAb. b A7 antigen expression in areas of higher cell density. c Reactivity of isotype control IgG on ROS17/2.8 CL#7 cells. Bars, 50 µm

Back to article page