Fig. 2
Comparison of trisomic and disomic iPS cells. a Growth rate of T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the cell number from three independent experiments. b Heat map and hierarchical clustering of the normalized gene expression values in T21-iPSC#12, D21-iPSC#1, D21-iPSC#2, and mixed-iPSCs for the genes on chromosome 21. Hierarchical clustering of genes using Multi-Experiment Viewer. c Hierarchical clustering of the normalized gene expression values in T21-iPSC#12, D21-iPSC#1, D21-iPSC#2, and mixed-iPSCs for all the genes. Hierarchical clustering of genes using Multi-Experiment Viewer. d Quantitative RT-PCR analysis for expression of APP in T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the expression from three independent experiments. *p < 0.05. e Quantitative RT-PCR analysis for expression of DYRK1A in T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the expression from three independent experiments. *p < 0.05. f Quantitative RT-PCR analysis for expression of DSCR1 in T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the expression from three independent experiments. *p < 0.05. g Quantitative RT-PCR analysis for expression of SOD1 in T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the expression from three independent experiments. *p < 0.05. h Quantitative RT-PCR analysis for expression of ETS2 in T21-iPSC#12 and D21-iPSC#2. Data shown are mean ± SD of the expression from three independent experiments. N.S., not significant
