Fig. 3 | Laboratory Investigation

Fig. 3

From: Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy

Fig. 3

Fluorescence staining of failing cardiac tissues with WFA and various molecular markers. Serial cardiac sections of 4C30 mice were stained separately with antibodies against Myl2 (ventricular cardiomyocyte marker), Cd31 (endothelial cell marker), Vim (fibroblast marker), and Col1a1/Col1a2 (major components of collagen fibers), and then stained with WFA. Left panels are fluorescence images of the perivascular regions of the triple-stained LV tissues for the indicated molecular marker (red), WFA (green), and Hoechst 33342 (blue). Right panels are enlarged images of the double staining for the marker (magenta) and WFA (green) at the indicated areas in the left panels. Scale bars, 50 µm

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