Fig. 5 | Laboratory Investigation

Fig. 5

From: TNFAIP2 expression induces epithelial-to-mesenchymal transition and confers platinum resistance in urothelial cancer cells

Fig. 5

Identification of MTDH as a mediator of TNFAIP2-derived EMT acquisition. a Microarray gene expression analysis revealed 19 genes (11 downregulated [blue] and 8 upregulated genes [red]) in 5637PR cells whose expression profiles were altered after si-TNFAIP2 transfection. Genes with an absolute change >2 or <0.5 from mock-transfected cells were identified as being differentially expressed. b mRNA expression of MTDH in 5637 and 5637PR cells. c Effects of TNFAIP2 knockdown and MTDH expression on 5637PR cells. d Effects of MTDH knockdown and E-cadherin and TWIST1 expression on 5637PR cells. e Matrigel invasion assay of 5637PR cells transfected with si-MTDH (n = 4). Box-and-whisker plots show medians (line within box), upper and lower quartiles (bounds of box), and minimum and maximum values (bars). *P < 0.05, from the Student’s t-test

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