Fig. 5 | Laboratory Investigation

Fig. 5

From: PI3K/Akt pathway-mediated HO-1 induction regulates mitochondrial quality control and attenuates endotoxin-induced acute lung injury

Fig. 5

Induction of HO-1 enhanced mitochondrial fusion whereas restrained fission. a, b The expression of protein and mRNA of the dynamic markers including Mfn2, OPA1, and Drp1 for mitochondrial fusion/fission in RAW264.2 cells. c The mitochondrial membrane potential (ΔΨm) determined by JC-1 probe. Graphs represented as mean ± s.d. of n = 6 independent experiments. Significant differences were indicated with an asterisk: *P < 0.05, **P < 0.01, ***P < 0.001. d, e The levels of Mfn2, OPA1, and Drp1 mRNA and protein in the lung tissue of rats. β-Actin served as a standard for protein loading. Values were expressed as mean ± s.d. of six individual samples using one-way ANOVA corrected with Bonferroni coefficient for multiple comparisons. *Significant difference from controls (P < 0.05). #Significant difference from LPS-treated cells or rats (P < 0.05). LPS lipopolysaccharide, HO-1 heme oxygenase-1, Mfn2 mitofusin 2, OPA1 optic atrophy 1, Drp1 dynamin-related protein 1, ΔΨm mitochondrial membrane potential, NS not significant

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