Fig. 6

Induction of HO-1 elevated dynamic changes of mitochondrial mitophagy. a, b Levels of key mediators of mitophagy, Parkin, and PINK1 were assessed by real-time PCR and western blots in RAW264.7 cells. c, d Representative protein or mRNA of Parkin and PINK1 expression in the lung tissues of rats. Data were presented as protein density relative to β-actin. The blots were representative of six independent experiments using one-way ANOVA corrected with Bonferroni coefficient for multiple comparisons. Values were expressed as mean ± s.d. (n = 6). ^*P < 0.05 vs. control and ^#P < 0.05 vs. LPS-treated vehicles. Significant differences were indicated with an asterisk: ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. LPS lipopolysaccharide, HO-1 heme oxygenase-1, PINK1 PTEN-induced putative kinase 1, NS not significant