Table 1 The PCR primer sequences and the length of fragments

From: PI3K/Akt pathway-mediated HO-1 induction regulates mitochondrial quality control and attenuates endotoxin-induced acute lung injury

Genes

Forward primer

Reverse primer

Length(bp)

HO-1

GAATCGAGCAGAACCAGCCT

CTCAGCATTCTCGGCTTGGA

135

Mfn2

ACTTCTCCTCTGTTCCAGTTGT

GTGCTTGAGAGGGGAAGCAT

181

OPA1

ACCTTGCCAGTTTAGCTCCC

ACCTAACAAGAGAAGGGCCTC

131

Drp1

GCCTCAGATCGTCGTAGTGG

TGCTTCAACTCCATTTTCTTCTCC

187

Tfam

ATGGGTGTTGGGAAGTCT

CTCTCCTGTTGGTGGCTC

168

NRF1

TCAGGGATAGGGGTTCA

TCAACCAGTTCGGCATA

157

PGC1α

AGTGAAGATGAAAGTGA

TTGAGAAAATAAGGATT

141

Parkin

CTGCGTGTGATTTTTGCC

AGGTGCTCTGGGGTTCGT

154

PINK1

GACCTCAAGTCCGACAACA

TGCCACCACGCTCTACAC

145

β-actin

TGTGTCCGTCGTGGATCTGA

TTGCTGTTGAAGTCGCAGGAG

149

  1. HO-1 heme oxygenase-1, Mfn2 mitofusin 2, OPA1 optic atrophy 1, Drp1 dynamin-related protein 1, Tfam mitochondrial transcription factor A, NRF1 nuclear respiratory factor 1, PGC1α peroxisome proliferator-activated receptor γ coactivator 1
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