Fig. 7
Immunohistochemical and immunofluorescence detection of the apoptotic nuclei in the testis of control and mutant mice. a Caspase-3 showed weak reaction in interstitial tissues (arrowhead) in WT mice. b Massive number of brown-stained apoptotic cells (arrowheads) was observed in all seminiferous tubules and interstitial tissues of mutant mice. c Occasional apoptotic cells (boxed areas) present in WT mice, especially in the developing spermatids (arrowhead). d The majority of germ cells from mutant mice are undergoing apoptosis (boxed areas). Note: fragmentation of the nuclei of Leydig cells (arrow)
