Fig. 1 | Laboratory Investigation

Fig. 1

From: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

Fig. 1

NanoSuit method for observing paraffin sections by scanning electron microscopy (SEM). a Workflow for correlative microscopy analysis of paraffin sections with the NanoSuit method. b On-demand droplet spotter system used to place marks around the region of interest using surface shield enhancer (SSE) solution. Droplets of undiluted SSE stock solution were identified as markers around the region of interest (black mark) and imaged using a light microscope (c) and a low-vacuum scanning electron microscopy (Lv-SEM) (d). e Image of a tissue section restained with hematoxylin and eosin after SEM observation and removing the SSE solution markings. The white bars shown in c–e represent 1 mm. f–h High charging effects of control sections without SSE solution (Lv-SEM in the secondary electron image). i–k SSE solution-coated sections showing a reduced charging effect (Lv-SEM in the secondary electron image). l, m field emission-scanning electron microscope (FE-SEM) secondary electron image of lung alveolar tissue. l NanoSuit-coated lung tissue. m Carbon-coated lung tissue. The scale bars represent 500 (f, i), 100 (g, j, l, m), and 50 μm (h, k)

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