Fig. 5 | Laboratory Investigation

Fig. 5

From: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

Fig. 5

Identifying 3,3′-diaminobenzidine (DAB)-stained regions using the NanoSuit method. a–g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f. Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm (a–c), 500 (d, e), 50 (f, g), and 10 μm (h–j)

Back to article page