Fig. 6 | Laboratory Investigation

Fig. 6

From: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

Fig. 6

Direct observation of gold particles in immunostained sections using the NanoSuit method. a–c Staining of a breast cancer section with a human epidermal growth factor receptor 2 (HER2) score of 3+. a HER2 expression detected by 3,3′-diaminobenzidine (DAB) staining (brown). HER2 expression observed by detection of 40-nm gold particle signals as multiple white dots in a low-vacuum scanning electron microscopy (Lv-SEM) image taken in backscattered electron (BSE) mode (b), or in an FE-SEM taken in secondary electron mode (c). Staining of a breast cancer section with a HER2 score of 0. No HER2 expression was detected by DAB staining (brown) (d), in an Lv-SEM image taken in BSE mode (e), or in an FE-SEM image taken in secondary electron mode (f). g, h Detection of human cytomegalovirus (CMV) with an anti-gB antibody conjugated with 40-nm gold particles. The white square in g was magnified and multiple 40-nm gold particles on the surface of human CMV particles are shown in h. i F-actin detected with 40-nm gold particles on TNT-like structures. The scale bars represent 200 (a, d), 20 (b, e), 2 (c, f, g, i), and 1 μm (h)

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