Fig. 7 | Laboratory Investigation

Fig. 7

From: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

Fig. 7

Energy-dispersive X-ray (EDX) analysis of paraffin sections. a–d Paraffin sections from patients with siderosis. a In hematoxylin and eosin (H&E) sections, putative iron-deposition areas (brown) were identified by light microscopy. b A clear backscattered electron (BSE) image (15 kV) was obtained without electric charge. Iron deposition (green, c) and phosphorous deposition (red, d) were observed by scanning electron microscopy (SEM)/EDX analysis. White arrows show the representative element deposition. e–k Anthracosis of the lung. With multiple foreign depositions (brown) observed in H&E-stained tissue (e). Many of depositions had a strong BSE signal (f). Aluminum (green, g), silicon (red, h), magnesium (yellow, i), oxygen (green, j), and carbon (purple, k) were analyzed by SEM/EDX. The scale bars represent 100 μm

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