Fig. 5

PCR assays from cells extracted from bone in vivo. Results in 8-week (shown) and 16-week-old mice were similar. N = 6, mean ± SD. Primers are shown in Table 1. Empty bones (after bone marrow was flushed for MSC isolation) were cut with razor blades, lysed, and processed for RNA isolation and quantitative PCR. a–e Osteoblast parameters. f–h Osteoclast parameters