Fig. 6
Effects of Scarb1 independent of ACTH demonstrated in vitro. a, b von Kossa, alkaline phosphatase, and Oil Red-O staining of wild type and Scarb1 knockout MSC differentiated into osteoblasts for 3 weeks in vitro. Two representative cultures of each type are shown. Differentiation of several mesenchymal stem cell preparations of each type was consistent. Whole wells of six-well plates are shown (3.5 cm). a Wild-type cells. b Scarb1 knockout cells. c Quantitative alkaline phosphatase in osteoblasts differentiated 3 weeks from MSC of wild-type and Scarb1 knockout cells. Mean ± SD, N = 4, p < 0.01. d Microscopic appearance of alkaline phosphatase, von Kossa, and Oil Red-O staining of wild type and Scarb1 knockout MSC differentiated into osteoblasts for 3 weeks in vitro. Fields are 200 microns wide. e Microscopic appearance of tartrate-resistant acidic phosphatase (TRAP) staining of osteoclasts differentiated from splenocytes of wild type and Scarb1 knockout for 10 days in vitro. Fields shown are 400 microns wide. Examples of osteoclasts are indicated with arrows. f The number of osteoclasts formed from splenocytes of Scarb1 KO mice and of wild-type mice
