Fig. 8 | Laboratory Investigation

Fig. 8

From: The high-density lipoprotein receptor Scarb1 is required for normal bone differentiation in vivo and in vitro

Fig. 8

Dose-dependent effect of ACTH on osteoblast and osteoclasts differerentiating. a–c Effects of 0, 1, 10, 100, and 1000 nM ACTH on wild type MSC differentiated into osteoblasts for 3 weeks. a Mineralization (von Kossa stain). Shown are 3 cm wide images of wells from a six-well plate. b Expression of osteocalcin by PCR. c Expression of alkaline phosphatase by PCR. d Effects of 0, 10, 100, and 1000 nM ACTH on wild-type splenocytes differentiated into osteoclasts for 10 days. Osteoclasts numbers from two different cultures were calculated

Back to article page