Table 2 Number of evaluable and overlapping cases for Allred and DIA scoring of SOX9, CDX2, SOX2, β-catenin, and E-cadherin available for comparisons
Biomarker | Evaluable cases | Overlapping cases DIA/Allred | IHC staining method |
|---|---|---|---|
CDX2 nucl (DIA) | 4-plex IF mIHC | ||
Min | 0.03 | ||
Max | 6.5 | ||
n (mean; sd) | 373 (1.9 ± 1.4) | 363 | |
CDX2 nucl (DIA)a | 5-plex IF mIHC | ||
Min | 0.001 | ||
Max | 7.4 | ||
n (mean; sd) | 814 (1.1 ± 1.1) | 589 | |
CDX2 nucl (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 642 (7.4 ± 1.7) | 363/589b | |
SOX2 nucl (DIA) | 4-plex IF mIHC | ||
Min | 0.006 | ||
Max | 23.6 | ||
n (mean; sd) | 373 (0.58 ± 1.88) | 361 | |
SOX2 nucl (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 645 (1.2 ± 2.6) | 361 | |
SOX9 nucl (DIA) | 3-plex IF mIHC | ||
Min | 0.13 | ||
Max | 5.3 | ||
n (mean; sd) | 181 (1.2 ± 1.0) | 172 | |
SOX9 nucl (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 761 (5.6 ± 1.5) | 172 | |
β-catenin nucl (DIA) | 3-plex IF mIHC | ||
Min | 0.045 | ||
Max | 34.5 | ||
n (mean; sd) | 380 (6.4 ± 5.8) | 321 | |
β-catenin nucl (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 637 (3.9 ± 2.0) | 321 | |
β-catenin cyto (DIA) | 3-plex IF mIHC | ||
Min | 0.1 | ||
Max | 21.2 | ||
n (mean; sd) | 380 (4.6 ± 3.7) | 361 | |
β-catenin cyto (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 722 (6.7 ± 1.3) | 361 | |
E-cadherin cyto (DIA) | 3-plex IF mIHC | ||
Min | 0.18 | ||
Max | 20.9 | ||
n (mean; sd) | 420 (5.1 ± 3.0) | 386 | |
E-cadherin cyto (Allred) | DAB chromogen + hematoxylin counterstain | ||
Min | 0 | ||
Max | 8 | ||
n (mean; sd) | 720 (6.6 ± 0.9) | 386 | |
