Fig. 3: Comparison of TRPC5 mRNA and protein expression in the adult retina.
From: TRPC5 regulates axonal outgrowth in developing retinal ganglion cells
a HEK293 cells expressing mouse TRPC5 + EGFP were stained by anti-GFP (green) and anti-TRPC5 (red) antibodies. The nuclei were visualized by Hoechst (cyan). The arrow indicates EGFP- and TRPC5-negative cell. The arrowheads indicate TRPC5 protein localized in the plasma membrane. b The expression pattern of TRPC5 mRNA by antisense probe (left) and sense probe (right) in the adult retina. c Immunostaining for TRPC5 (green) and Hoechst (cyan) in the adult retina. d Immunostaining by anti-rabbit IgG antibody (green) and Hoechst (cyan) in the adult retina. Scale bar: 5 µm (a); 20 µm (b–d). GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, ONL outer nuclear layer.
