Fig. 1: O-GlcNAcylation in pancreatic cancer cells with different sensitivity to TRAIL-induced apoptosis.
From: Regulation of pancreatic cancer TRAIL resistance by protein O-GlcNAcylation
A  TRA-8-induced apoptosis. Pancreatic cancer cells, BxPC-3, MiaPaCa-2, PANC-1, and S2VP10, were exposed to TRA-8 (1 μg/ml) for 24 h; and apoptotic cells were detected by flow cytometry. Results shown are means ± SD of three independent experiments performed in duplicate. B O-GlcNAcylation in pancreatic cell lines, as determined by western blot analysis using specific antibodies for, DR5, OGT, and O-GlcNAcylation. The expression of ß-actin was used as a loading control. Representative blots from three independent experiments are shown.
