Fig. 5: MXI1-NUTM1 effect on cell proliferation in vitro. | Laboratory Investigation

Fig. 5: MXI1-NUTM1 effect on cell proliferation in vitro.

From: A MXI1-NUTM1 fusion protein with MYC-like activity suggests a novel oncogenic mechanism in a subset of NUTM1-rearranged tumors

Fig. 5: MXI1-NUTM1 effect on cell proliferation in vitro.

a Immunoblots with anti-hemagglutinin (HA), MYC, HRAS, and β-actin (loading control) antibodies on cell lysates from NIH-3T3 cells transduced with combinations of empty vector (EV), HA-MXI1-NUTM1, MYC, and oncogenic HRAS expressing retroviruses. A duplicate HA-MXI1-NUTM1-only lane has been cropped from the immunoblots for clarity. ATP abundance (b Cell Titer Glo assays) and cell confluency (c Incucyte assay) in NIH-3T3 cells transduced with either empty vector (EV), HA-MXI1-NUTM1 or MYC (1% serum conditions). All data are expressed as the mean + SD.

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