Fig. 6: MXI1-NUTM1 effect on anchorage-independent growth in vitro. | Laboratory Investigation

Fig. 6: MXI1-NUTM1 effect on anchorage-independent growth in vitro.

From: A MXI1-NUTM1 fusion protein with MYC-like activity suggests a novel oncogenic mechanism in a subset of NUTM1-rearranged tumors

Fig. 6: MXI1-NUTM1 effect on anchorage-independent growth in vitro.

NIH-3T3 cells transduced with oncogenic HRAS (from a HRAS-IRES-GFP vector) and either empty vector (EV), HA-MXI1-NUTM1, or MYC. a Cells grown on plastic substrate in 10% serum, showing box and whisker plots of colony number and representative images of colonies (visualized by GFP fluorescence). b Cells grown in soft agar, 10% serum. Box and whisker plots for colony number, area per colony and total colony area. c Images of colonies from cells grown in soft agar, 10% serum (top row) and IHC for NUTM1 and MYC on paraffin blocks of cells grown on plastic in 10% serum. Statistical comparisons were made using unpaired two-tailed t-tests. Data were analyzed using GraphPad Prism 8.1.0. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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