Fig. 1: NO depletion with c-PTIO abolishes the capacity of breast cancer cells to form mammospheres in vitro. | Laboratory Investigation

Fig. 1: NO depletion with c-PTIO abolishes the capacity of breast cancer cells to form mammospheres in vitro.

From: Nitric oxide-targeted therapy inhibits stemness and increases the efficacy of tamoxifen in estrogen receptor-positive breast cancer cells

Fig. 1

a Representative images and percentage of mammospheres formed after 2 weeks of seeding MCF-7 cells pre-treated during 24 h with the indicated doses of c-PTIO. b Immunoblot of Notch-1 in nuclear fractions of MCF-7 cells. Cells were treated with different concentrations of c-PTIO for 24 h and Notch-1 was detected using a specific antibody. Transcription factor II-B (TFIIB) was used as loading control. c Representative images and mammospheres percentage formed from MCF-7, MDA-MB-361 and BT-474 cells pre-treated for 24 h with c-PTIO (100 μM), in the absence or the presence of the NO-donor DETANONOate (100 μM). Scale bars: 50 μm. Data are means ± SEM of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001 compared with control).

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