Fig. 3: NOS2 deficiency inhibits mammosphere formation and the expression of CSCs-specific markers in ER+ breast cancer cells. | Laboratory Investigation

Fig. 3: NOS2 deficiency inhibits mammosphere formation and the expression of CSCs-specific markers in ER+ breast cancer cells.

From: Nitric oxide-targeted therapy inhibits stemness and increases the efficacy of tamoxifen in estrogen receptor-positive breast cancer cells

Fig. 3

a Representative images and percentage of mammospheres formed by shRNA-control and shRNA-NOS2 MCF-7 cells. Scale bars: 50 μm. b Notch-1 protein expression levels in shRNA-control and shRNA-NOS2 MCF-7 cells. Stain-free technology was used as loading control. c Analysis by flow cytometry of CD44+/CD24−/low subpopulation in shRNA-control and shRNA-NOS2 MCF-7 cells. d Analysis of CD44 mRNA levels in shRNA-control and shRNA-NOS2 MCF-7 cells. Data are means ± SEM of three independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared with control).

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