Fig. 3: Stress-elevated hepatic CYP7A1 protein levels via corticosterone in mice. | Laboratory Investigation

Fig. 3: Stress-elevated hepatic CYP7A1 protein levels via corticosterone in mice.

From: Stress can attenuate hepatic lipid accumulation via elevation of hepatic β-muricholic acid levels in mice with nonalcoholic steatohepatitis

Fig. 3

A Serum corticosterone levels after challenge with short STR (short stress). Significance was determined by t test. (***P < 0.001). B Quantitative PCR analysis of Cyp7a1, Slc51b, and Abcc4 gene expression. Data are presented as the fold change relative to the value for the non-stress group. C Western blot analysis of hepatic CYP7A1 protein after short stress challenge. The signal intensity was measured using ImageJ. Significance was determined by t test. (**P < 0.01). D Western blot analysis of CYP7A1 protein (6 and 24 h) and quantitative PCR analysis for Cyp7a1 gene expression in mouse primary hepatocytes at 6 h after treatment with 300 nM corticosterone. Data are presented as the fold change relative to the value for the vehicle. Each bar represents the mean and SD (n = 3). Significance was determined by unpaired Student’s t test (**P < 0.01 for the vehicle vs. corticosterone). E Cyp7a1 gene expression analysis in mouse primary hepatocytes at 6 h after treatment with corticosterone in a dose-dependent manner. (Upper panel; western blot analysis, bottom panel; quantitative PCR analysis).

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