Fig. 3: PAI-1 promotes the migration and invasion of esophageal squamous cell carcinoma (ESCC) cells by activating Akt and Erk1/2 signaling pathways. | Laboratory Investigation

Fig. 3: PAI-1 promotes the migration and invasion of esophageal squamous cell carcinoma (ESCC) cells by activating Akt and Erk1/2 signaling pathways.

From: PAI-1 derived from cancer-associated fibroblasts in esophageal squamous cell carcinoma promotes the invasion of cancer cells and the migration of macrophages

Fig. 3

a Levels of LRP1 mRNA in ESCC cells by RT-PCR. GAPDH used as internal control. b Expression of LRP1 in ESCC cells by western blotting. β-actin used as the loading control. c Transwell assay of the effects of recombinant human (rh) PAI-1 on the migration of TE-8, TE-9, and TE-15 cells. Migrating cells were counted in four randomly chosen fields. d Transwell assay of the effects of rhPAI-1 on the invasion of TE-8, TE-9, and TE-15 cells. Invading cells were counted in four randomly chosen fields. e Representative western blots of phosphorylated and total Akt and Erk1/2 in ESCC cells. TE-8, TE-9, and TE-15 cells in serum-free conditions were treated with 10 ng/mL rhPAI-1 for 0, 10, 30, and 60 min. f Transwell migration assay of TE-8, TE-9, and TE-15 cells with or without 10 ng/mL rhPAI-1 combined with an inhibitor against PI3K (LY294002, 10 μM) or MEK1/2 (PD98059, 10 μM). Migrating cells were counted in four randomly chosen fields. g Invasion assay of TE-8, TE-9, and TE-15 cells with or without 10 ng/mL rhPAI-1 combined with an inhibitor against PI3K (LY294002, 10 μM) or MEK1/2 (PD98059, 10 μM). Invading cells were counted in four randomly chosen fields. For (c), (d), (f), and (g), data represent the mean ± SEM of triplicate wells for three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).

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