Fig. 4: MiR-1246 and miR-10b-5p promote glioma cell migration and invasion in vitro and in vivo. | Laboratory Investigation

Fig. 4: MiR-1246 and miR-10b-5p promote glioma cell migration and invasion in vitro and in vivo.

From: Exosomes derived from hypoxic glioma deliver miR-1246 and miR-10b-5p to normoxic glioma cells to promote migration and invasion

Fig. 4

a, b Analysis of glioma cell migration and invasion by transwell assay. a MiR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG and U251 glioma cells that migrated to (without Matrigel coating) or invaded (with Matrigel coating) the bottom surface were stained with crystal violet and observed by light microscopy. Scale bar, 100 μm. b Quantitative analysis of miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG and U251 glioma cells that migrated to or invaded the bottom surface. c Analysis of glioma invasion by 3D invasion assay. Representative images of invaded spheroids in the 3D invasion assay for miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG and U251 glioma cells. Scale bar, 200 mm. d Quantitative analysis of area covered by miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG and U251 glioma cells. e Western blot analysis of N-cadherin, MMP2, MMP9, and vimentin in miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG and U251 glioma cells. f In vivo bioluminescent imaging of miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG glioma cells implanted in situ in nude mice. Representative images and quantification (25 days after implantation) are shown (five mice in each group). g HE staining of sections from mouse brain xenografts with miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG glioma cells. Scale bar, 200 μm. h Survival analysis for nude mice bearing miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG glioma cell xenografts (P < 0.05 by log-rank analysis). i Quantitative analysis of IHC staining for N-cadherin and MMP2 of sections from mouse brain xenografts with miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG glioma cells. j Quantitative analysis of IHC staining for Ki67 of sections from mouse brain xenografts with miR-NC-, miR-1246-, and miR-10b-5p-transfected U87MG glioma cells. Data are shown as the mean ± SD of three independent experiments. Statistical significance was determined using Student’s t test, ANOVA test and log-rank analysis (*P < 0.05; **P < 0.01).

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