Fig. 1: IL-17 enhanced TGF-β1 expression. | Laboratory Investigation

Fig. 1: IL-17 enhanced TGF-β1 expression.

From: TGFβ1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial–mesenchymal transition in Sjögren’s syndrome

Fig. 1: IL-17 enhanced TGF-β1 expression.The alternative text for this image may have been generated using AI.

TGF-β1 gene and protein expression was quantified by semiquantitative RT-PCR (a), real-time PCR (c), western blotting (f), and ELISA (d) in in vitro cultured healthy SGEC treated with IL-17 for 24/48 h and in untreated control healthy SGEC. The images show that IL-17 treatment determined an increased TGF-β1 gene and protein expression. Band intensities were analyzed by densitometric analysis performed by gel image software, normalized against that of GAPDH for RT-PCR, and β-actin for western blotting, and expressed in arbitrary units (b, e). In all experimental procedures pSS SGEC were used as positive control. (Data represent mean ± SE; n = 3). Asterisks and arrowheads indicate statistical significance p < 0.01. M Marker.

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