Fig. 5: ELK-1 /ZC3H4 regulates EMT and TGF-β production. | Laboratory Investigation

Fig. 5: ELK-1 /ZC3H4 regulates EMT and TGF-β production.

From: Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial–mesenchymal transition

Fig. 5

a Representative western blots showing the effects of CRISPR/Cas9-mediated ZC3H4 silencing (ZC3H4 NIC) on EMT and TGF-β production induced by ELK-1 ACT in MLE-12 cells. b Densitometric analyses of five independent experiments showing that CRISPR/Cas9-mediated ZC3H4 silencing attenuates the ELK-1 ACT-induced increase in Col I expression and TGF-β production, while ameliorates the ELK-1 ACT-induced decrease in E-cad expression; *p < 0.05 vs. the control group; #p < 0.05 vs. the ELK-1 ACT group. c Representative immunocytochemistry images demostrating that ELK-1 ACT induces the expression of E-cad and increased the expression of Col I. CRISPR/Cas9-mediated ZC3H4 silencing attenuates the ELK-1 ACT-induced EMT in MLE-12 cells. Scale bar = 20 μm.

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