Fig. 7: OS is involved in EMT via ELK-1 later expression. | Laboratory Investigation

Fig. 7: OS is involved in EMT via ELK-1 later expression.

From: Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial–mesenchymal transition

Fig. 7

a Quantitative results for intracellular ROS levels in MLE-12 cells are determined by relative fluorescence intensity; n = 5; *p < 0.05 vs. the control group. b SiO2 downregulates SOD activity (U/mg pro) and GSH/GSSG (c); n = 5; *p < 0.05 vs. the control group. MLE-12 cells are pretreated with NAC (1 mM) for 1 h before SiO2 stimulation for 24 h (d–g). d Representative western blots showing NAC inhibits SiO2-induced increase in ZC3H4, Col I, and TGF-β expressions, while reverses the SiO2-induced decrease in E-cad expression. e Densitometric analyses of the ZC3H4, Col I, TGF-β, and E-cad expression (n = 5); *p < 0.05 vs. the control group. #p < 0.05 vs. the SiO2 group. f Representative western blots showing the downregulated phosphorylation of Elk-1 in the nucleus of MLE-12 cells pretreatmented with NAC. g Densitometric analyses of Elk-1 phosphorylation (n = 5); *p < 0.05 vs. the control group. #p < 0.05 vs. the SiO2 group.

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