Fig. 1: ASIC1a is highly expressed in RA synovial tissue. | Laboratory Investigation

Fig. 1: ASIC1a is highly expressed in RA synovial tissue.

From: Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a

Fig. 1

a Immunohistochemical visualization of ASIC1a in normal human synovial tissue and synovial tissue in RA patients (normal: n = 3, RA: n = 25) ***P < 0.001 versus normal group. b, c Detection of CD55 and ASIC1a in RASF by flow cytometry. d Western blotting analysis of ASIC1a and 2,3,4 protein expression in RASF. Histograms show semiquantitative analysis of gels from western blotting. e Western blotting analysis of ASIC1a protein expression in RASF membrane, histograms show semiquantitative analysis of gels from western blotting. Western blotting bands represent three independent experiments. Data are expressed as mean ± SD, *P < 0.05 versus NSF group, **P < 0.01 versus NSF group, ***P < 0.001 versus NSF group. f Representative photomicrographs of RASF were stained to detect ASIC1a (green fluorescence) and nucleus (DAPI, blue fluorescence). The scale bar represents 20 mm (To explain the references to colors in this legend, see the web version of this article.).

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