Fig. 2: ASIC1a regulates the release of VEGF by RASF. | Laboratory Investigation

Fig. 2: ASIC1a regulates the release of VEGF by RASF.

From: Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a

Fig. 2

a Elisa for cell culture supernatants VEGF, **P < 0.01 versus 0 h group. b Western blotting analysis of VEGF protein expression in RASF. Histograms show semiquantitative analysis of gels from western blotting. Western blotting bands represent three independent experiments. Data are expressed as mean ± SD, **P < 0.01 versus 0 h group, ***P < 0.001 versus 0 h group. c Representative photomicrographs of RASF were stained to detect VEGF (green fluorescence) and nucleus (DAPI, blue fluorescence). The scale bar represents 20 mm. d ASIC1a protein expression levels were decreased in RASF by shRNA treatment. The silencing of ASIC1a in RASF was analyzed by western blotting. Histogram showing the semiquantitative analyses of the gels from western blotting. Western blot bands are representative of three independent experiments. Data were expressed as the mean ± SD, **P < 0.01 versus RASF group. e, f Elisa for cell culture supernatants VEGF after ASIC1a silencing or blocking, **P < 0.01 versus RASF group. g Markedly increased levels of the ASIC1a protein were detected by western blotting in RASF transfectants. Histogram showing the semiquantitative analyses of the gels from western blotting. Western blot bands are representative of three independent experiments. Data were expressed as the mean ± SD, ***P < 0.001 versus RASF group. h Elisa for cell culture supernatants VEGF after ASIC1a is overexpressed, **P < 0.01 versus RASF group.

Back to article page