Fig. 1: Suppression of miR-181a-5p attenuated the epilepsy-induced dysfunction of spatial learning and memory. | Laboratory Investigation

Fig. 1: Suppression of miR-181a-5p attenuated the epilepsy-induced dysfunction of spatial learning and memory.

From: Inhibition of miR-181a-5p reduces astrocyte and microglia activation and oxidative stress by activating SIRT1 in immature rats with epilepsy

Fig. 1

a qRT-PCR measurement of miR-181a-5p in the hippocampal tissues from the control rats and the rats subjected to SE and in vivo transfection at day 3 and day 48 (n = 6 per group). b The protein level of SIRT1 detected by Western blot in the hippocampal tissues in the control and epileptic rats. c The SIRT1 mRNA level detected by qRT-PCR (n = 6). d The binding of miR-181a-5p to SIRT1 was identified using a luciferase reporter gene assay in 293T cells. The relative luciferase activity was normalized and is shown as the mean ± SD (n = 3). e Escape latency of the different animal groups during the 5 training days in the MWM test. The data are shown as the mean ± SD based on n = 6. f The probe test was performed after 5 days of training, and the number of platform crossings was counted (n = 6 each group). The statistically significant differences in (a–f) were analyzed by one-way ANOVA with Tukey’s test for multiple comparisons (**P < 0.01).

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