Fig. 4: HNRNPA2B1 promotes epithelial-to-mesenchymal transition. | Laboratory Investigation

Fig. 4: HNRNPA2B1 promotes epithelial-to-mesenchymal transition.

From: The HNRNPA2B1–MST1R–Akt axis contributes to epithelial-to-mesenchymal transition in head and neck cancer

Fig. 4: HNRNPA2B1 promotes epithelial-to-mesenchymal transition.The alternative text for this image may have been generated using AI.

a Immunoblotting of Flag-tagged HNRNPA2 in HNRNPA2B1-depleted H413 cells. b Semiquantitative PCR analysis of alternative splicing of MST1R upon HNRNPA2B1 depletion as well as, upon ectopic expression of Flag-tagged HNRNPA2 in HNRNPA2B1-depleted H413 cells. c MST1R-minigene transfection and fluorescence analysis in control cells, HNRNPA2B1-depleted with vector control cells, and HNRNPA2B1-depleted HNRNPA2 expressing HNC cells. Scale bar = 200 µm. d Immunoblotting of HNRNPA2B1, pAkt, pan Akt, E-cadherin, vimentin, and GAPDH in control cells, HNRNPA2B1-depleted and HNRNPA2B1-depleted HNRNPA2 expressing H413 cells. e Relative cellular proliferation analyzed using MTT assay in HNRNPA2B1-depleted and HNRNPA2 reexpressing H413 cells (n = 3). f The cell invasion was analyzed via Matrigel invasion assay, (left) single field of invaded cells was captured under the microscope, scale bar: 250 µm, and (right) invaded cells were counted at five different fields under the microscope (n = 3).

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