Fig. 2: sCLs contained LGR5-positive cells with high frequency. | Laboratory Investigation

Fig. 2: sCLs contained LGR5-positive cells with high frequency.

From: Continuous formation of small clusters with LGR5-positive cells contributes to tumor growth in a colorectal cancer xenograft model

Fig. 2

a Distribution of LGR5-positive cells in each 3D structure. Number shows the order of serial sections. Tumor cells are enclosed by dotted line. LGR5 (green) and HLA-DMA (red). White arrows indicate part of small lumen in gland. Scale bars = 25 μm for sCLs and 50 μm for glands. b Relationship between frequency of LGR5-positive cells and maximum external and internal diameter of sCLs. LGR5-positive grade: rare: white; occasional: blue; moderate: yellow; frequent: green. c Immunofluorescence for LGR5 (green)/E-cadherin (red), β-catenin (red), and LGR5 (green)/Ki67 (red) in sCLs and glands. Tumor cells are enclosed by dotted line. White arrows indicate bud structures in gland. Scale bar = 25 μm.

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